BPC-157 Reconstitution Calculator

This calculator of reconstitution of BPC-157 tells you how much concentration your solution has after combining the lyophilized peptide powder with bacteriostatic water. You need to provide the vial size along with the volume of water you are likely to put in the solution, and it provides you the amount of peptide solution per 0.1 ml. The calculator has been built with the purpose of simplify the reconstitution.

How the BPC-157 Reconstitution Calculator Works

The calculator uses a basic equation. It simply converts the milligram count of the vial to microgram units, divides that by the total water volume and multiplies that number by 0.1 to produce the concentration for each tick on a standard insulin syringe. There is no rounding or anything tricky about this calculation. We show you all the arithmetic step by step so you can check it against your own calculations.

Enter Your Vial Size in Milligrams

Check the label on your BPC-157 vial for the peptide quantity. The two most common sizes sold by US vendors are 5 mg and 10 mg. PeptideGod sells a 5 mg lyophilized vial at $34.95, and Real Peptides offers a 10 mg vial at $43.20 with published HPLC purity of 99% or higher and a lot-specific COA. Enter whichever milligram value matches your vial.

Enter the Volume of Bacteriostatic Water

This is the volume of the solvent that you will inject into the peptide vial. The common choice for multi-draw reconstitution is bacteriostatic water with 0.9% benzyl alcohol. Real Peptides offers a USP-grade 10 ml vial for $4.80 (SKU: RP-BAC10V). The amount of liquid used for reconstitution usually ranges between 1 ml and 3 ml. A greater amount of liquid reduces the concentration, while a lower amount increases it but keeps the total amount of peptide constant.

Read the Concentration per Unit

The results come in micrograms per 0.1 ml. A 1 ml insulin syringe with 100-unit marks has small dashes representing 0.01 ml each and 10 dashes equaling 0.1 ml. Once the concentration per 0.1 ml is determined, the amount of peptide in any draw volume can be calculated using simple multiplication. A calculator will do the work, but basically, the math is easy to confirm on paper.

Common Reconstitution Scenarios

The table presents us with the five combinations we come across most frequently. All values in the table presuppose full dissolution of the peptide without any loss of material during the process of mixing.

It is easy to understand the calculations of every row. 5 mg vial has 5000 mcg of the peptide in it. So if we divide 5000 mcg by 1 ml, we get 5000 mcg/ml and so each 0.1 ml is equivalent to 500 mcg. Then, in 2 ml vial, we get 2500 mcg/ml, which means every 0.1 ml is equal to 250 mcg. The same explanation applies for 10 mg rows too. In this case, we divide 10,000 mcg by 3 ml and so conclude that the calculation shows that 3333.3 mcg/ml is equal to 333.3 mcg/0.1 ml.

What Reconstitution Means for Lyophilized Peptides

Lyophilized is another term that refers to freeze-dried. The peptide solution is first frozen, and then a vacuum is created, leading to the sublimation of the ice. Instead of having to keep the peptide liquid, the whole process of lyophilization helps keep the integrity of the peptide and has a longer shelf life. You can find more insight in our guide on why lyophilization matters for peptide quality and shipping.

To apply the peptide, you simply do the opposite of what you previously did. Take the bacteriostatic water and draw the required amount into a syringe, then push the needle inside of the vial through the rubber stopper and add the liquid into the vial gently down the wall of the vial. Never apply the water directly on the powder. Allow the vial to sit for a few seconds, then tilt and gently move it around until the solution is clear. Do not shake the vial.

Storage After Reconstitution

The shelf life of BPC-157 depends entirely on whether the vial has been opened and what solvent was used. Unreconstituted lyophilized powder stored in a sealed vial at -20 degrees Celsius remains stable for 24 to 36 months. At refrigerator temperatures, the sealed powder holds for several months but degrades faster than in a freezer. For a full breakdown of temperature ranges, see our BPC-157 storage temperature guide.

Once reconstituted with bacteriostatic water, the solution should be refrigerated at 2 to 8 degrees Celsius and used within 28 to 60 days. The 0.9% benzyl alcohol in bacteriostatic water inhibits bacterial growth in the vial between draws, but it does not sterilize the solution or prevent peptide degradation indefinitely. A 2018 review of BPC-157 stability in preclinical models noted the peptide's resistance to degradation under various conditions, though reconstituted storage was not the primary focus of the study (Sikiric et al., 2018).

If you notice any cloudiness, particulates, or color changes in the solution, you should throw it away. Also, a reconstituted vial must never be frozen again. The freezing-thawing cycle will always result in the irreversible aggregation of peptides.